Analysis of α - particle induced incomplete chromosome aberrations , using pan - centromeric and pan - telomeric DNA probes
نویسندگان
چکیده
The aim of the present study has been the evaluation of the incompleteness of αparticle induced chromosome aberrations by the simultaneous detection of all centromeres and telomeres present in human lymphocytes. For this purpose attached lymphocytes were irradiated at doses of 0.2, 0.5, 0.7 and 1 Gy in a Am source. FISH techniques were applied using pan-centromeric and pan-telomeric probes. All abnormal cells were digitalised and analysed using a Cytovision® FISH workstation. A total of 378 incomplete chromosomes plus incomplete acentrics was found. Cases with more than 92 telomeres were not detected. The ratio between total incomplete elements and multicentrics was 1.00. The total number of acentric fragments was 822; 57% of them were complete fragments ace (+,+), 26% incomplete fragments ace (+,-), and 17% interstitial fragments ace(-,-). The percentage of incomplete aberrations is higher after high-LET than that described for low-LET exposures. The results seem to indicate that compared to low-LET, after α-particle exposure it is more likely to repair the centromere-containing elements.
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